Showing results for: [ Proteomics and Intermolecular Interactions (excl. Medical Proteomics) ]
Discovery proteomics data and quantitative proteomics data acquired on 15 commercial wheat cultivars and 8 MAGIC populations.
OCE-NCGS-PDF-Utpal Bose - - Published 06 Feb 2020
These collection is comprised of mass spectral datasets obtained from proteomics analysis of shrimp haemolymph used to resolve the complexity of hemocyanin isoforms
Ridley Alliance - - Published 06 Jan 2020
These files contain experimental data used to measure recombinant cytokines in bovine sera
MLA Heat Stress - - Published 05 Dec 2019
SWATH-MS acquisition was used to analyse the hordein-null barley lines. This dataset was investigated in order to understand the global proteome changes upon deletion of each hordein-class.
Legacy data - - Published 21 Aug 2019
Command-line Python program for the automated analysis of Hydropathic, Order-promoting, Short Linear Motifs (HO-SLiMs) in proteins.
SIP 321: Agriculture & Food Data School - Analysis of Protein Sequences - Published 13 Aug 2019
LC-MS/MS discovery characterisation of 20 rye cultivars after gluten enrichment (using IPA/DTT) and tryptic digestion.Data set includes raw MS data and ProteinPilot results files. The samples were als... moreo analysed with a quantitative LC-MRM-MS method (rye peptide markers) with all raw data uploaded for: (1) 20 rye cultivars; (2) 16 cereals and pseudocereals; (3) a range of food products (10 breakfast cereals and 7 snack foods); (4) repeat analysis on spelt after contamination was noted in batch 1.less
Livestock - Testing & Services - - Published 31 May 2019
Proteomics data collected as part of the manuscript by Tanner et al (2019) Frontiers in Plant Science
Abstract: The temporal pattern of accumulation of hordein storage proteins in developing barley gr... moreains was studied by enzyme-linked immunosorbent assay (ELISA), western blot and liquid chromatography tandem mass spectrometry (LC-MS/MS). Hordein accumulation was compared to the pattern seen for two abundant control proteins, serpin Z4 (an early accumulator) and lipid transferase protein (LTP1, a late accumulator). Hordeins were detected from six days post-anthesis (DPA) and peaked at 30 DPA. Changes in fresh weight indicate that desiccation begins at 20 DPA and by 37 DPA fresh weight had decreased by 35%. ELISA analysis of hordein content, expressed on a protein basis, increased to a maximum at 30 DPA followed by a 17% decrease by 37 DPA. The accumulation of 39 tryptic and 29 chymotryptic hordein peptides representing all classes of hordein was studied by LC-MS/MS. Most peptides increased to a maximum at 30 DPA, and either remained at the maximum or did not decrease significantly. Only five tryptic peptides, members of the related B1- and γ1-hordeins decreased significantly by 21-51% at 37 DPA. Thus, the concentration of some specific peptides was reduced while remaining members of the same family were not affected. The N-terminal signal region was removed by proteolysis during co-translation. In addition to a suite of previously characterised hordeins, two novel barley B-hordein isoforms mapping to wheat low molecular weight glutenins (LMW-GS-like B-hordeins), and two avenin-like proteins (ALPs) sharing homology with wheat ALPs, were identified. These identified isoforms have not previously been mapped in the barley genome. Cereal storage proteins provide significant nutritional content for human consumption and seed germination. In barley, the bulk of the storage proteins comprise the hordein family and the final hordein concentration affects the quality of baked and brewed products. It is therefore important to study the accumulation of hordeins as this knowledge may assist plant breeding for improved health outcomes (by minimizing triggering of detrimental immune responses), nutrition and food processing properties. less
Livestock - Testing & Services - - Published 03 Apr 2019