Showing results for: [ Microbiology not elsewhere classified ]
16S sequencing of hydrocarbon contaminated soil.
Spatio-temporal feedbacks between soil contamination and biotic responses - - Published 15 Oct 2019
Here we present the data of faecal indicator bacteria and opportunistic pathogen in roof-harvested rainwater tank samples in Queensland, Australia. The data on concentrations of opportunistic pathogen... mores in RHRW will provide information for rainwater users regarding potential seasonality of risks. Quantitative data presented in this study can be used to perform quantitative microbial risk assessment (QMRA) of RHRW for various potable and nonpotable uses. Data can be used by health regulators to develop guidelines related to RHRW. less
Water quality monitoring for SEQwater - Seasonal Assessment of Opportunistic Premise Plumbing Pathogens in Roof-Harvested Rainwater Tanks - Published 12 Jan 2017
The red macroalga Asparagopsis taxiformis has been shown to significantly decrease methane production by rumen microbial communities. This has been attributed to the bioaccumulation of halogenated met... morehane analogues produced as secondary metabolites. The objective of this study was to evaluate the impact of A. taxiformis supplementationon the relative abundance of methanogens and microbial community structure using an in vitro rumen fermentation model. Addition of A. taxiformis (2% organic matter) or the halogenated methane analogue bromoform (5 µM) reduced methane production by over 99% compared to a basal substrate only control. Quantitative PCR confirmed that the decrease in methane production was correlated with a decrease in the relative abundance of methanogens. High throughput 16S ribosomal RNA gene amplicon sequencing showed that both treatments reduced the abundance of the three main orders of methanogens present in ruminants (Methanobacteriales, Methanomassiliicoccales and Methanomicrobiales). Shifts in bacterial community structure due to the addition of A. taxiformis and 5 µM bromoform were similar and concomitant with increases in hydrogen concentration in the head space of the fermenters. With high potency and broad-spectrum activity against the diversity of rumen methanogens, A. taxiformis represents a promising natural strategy for reducing enteric methane emissions from ruminant livestock.less
Metagenomic approaches for understanding - In vitro response of rumen microbiota to the antimethanogenic red macroalga Asparagopsis taxiformis - Published 02 Jan 2017