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Showing results for: [ Medical Biochemistry: Nucleic Acids ]
We describe two versions of COBRA-seq: First, Genome-Wide COBRA (GW-COBRA) that uses PCR to exponentially amplify the sequencing library fragments after linker ligation. Second, Linear Amplification C... moreOBRA (LA-COBRA) that relies on T7 RNA polymerase-mediated transcription yielding many RNA transcripts of the library fragments generated in a linear manner which are subsequently converted to cDNA. The COBRA-seq library protocol is largely adapted to provide robust coverage of the human genome, however, all of the steps are applicable to both model and non-model organisms.
SIEF: Nutritional Genomics - COBRA-seq: Sensitive and Quantitative Methylome Profiling - Published 29 Sep 2015