Showing results for: [ Chariton, Anthony ]
18S rDNA, 16S rDNA, and diatom 18S v4 rDNA amplicons from sediment DNA collected from Vavouto Bay New Caledonia.
NiPERA - Metabarcoding reveals changes in benthic eukaryote and prokaryote community composition along a tropical sediment nickel gradient - Published 06 Jul 2020
The data describes the microbiome community of the coral Acropora mucricata following exposure to nickel and copper (separately) for 4 days under controlled laboratory conditions
NiPERA - The effect of dissolved copper and nickel on the adult coral Acropora muricata and its microbiome - Published 16 Apr 2019
Outdoor freshwater mesocosms spiked with varying concentrations of copper were sampled in order to profile microbial communities. Samples collected include water, sediment and cellulose baits. DNA was... more extracted from all sample types. In addition to this, RNA was extracted from sediment samples and reverse transcribed to get cDNA. The v4 region of the 16S ribosomal RNA gene was amplified using Earth Microbiome primers 515F and 806R.
The 16S rRNA gene amplicons were sequenced on the Illumina MiSeq platform with 250 base pair-end reads. Forward and reverse read alignments, quality filtering, chimera checking and OTU clustering were performed using USEARCH v9.
OTU sequences were submitted to GenBank, however, 130 were found to be too novel for submission and thus are made available here.less
Legacy data - Microbial community survey - Published 10 Oct 2017
Metabaroding data (16S/18S) examining the composition of communities sampled from bore access wells and the aquifer.
Acquired - Groundwater - Published 26 Jun 2016
16S rRNA gene amplification and sequencing were performed to assess the effects of uranium on sediment microbial communities. A total of 12 treatments along a uranium concentration gradient of 0 - 4,0... more00 mg/kg were interrogated, with four replicates per treatment. Data here include: raw MiSeq sequences (paired-end 250 base reads) as well as a mapping file for demultiplexing and a fasta file containing sequences of OTUs not deposited at DDBJ/ENA/GenBank (due to poor sequence similarity with existing entries). less
Estuarine Health Assessment - Next generation sequencing - Published 13 May 2016
Bacterial survey of the S Alligator River Floodplain (Kakadu).
The study was designed to examine how history to saltwater exposure alters microbial assemblages.
The research was part of the NERP Nort... morehern Australian Hub.
Data includes 90 samples taken from three different areas of energy (low, medium and high) and three areas of the floodplain (lower, upper and backwaters).
The data includes processed 16S rDNA amplicons, GPS co-ordinates and soil chemistry.
1177.2 Estuarine Health Assessment - Experiment - Published 01 Apr 2015
The data encompasses a benthic survey of the East and South Alligator Rivers, Kakadu National Park. Each estuary was survey on two occasions (wet and dry), encompassing eight sites from the mouth to t... morehe road crossings.
1177.2 Estuarine Health Assessment - Experiment - Published 27 Mar 2015
eDNA metabarcoding was performed to assess the effects of copper on the establishment of the benthic eukaryote community within a series of environmentally relevant copper contaminated fresh... morewater mesocosms. The data is obtained from a recolonization experiment contained five treatments : control (C) and four sediment copper concentrations (very low (VL), low (L), high (H) and very high (VH)), each with four replicates. The data represents samples obtained on seven occasions subsequent to the experiment being established: 15, 31, 135, 161, 365, 407 and 497 days.
Details on the experimental design are available in:
Gardham, S., G. C. Hose, S. L. Simpson, C. Jarolimek, and A. A. Chariton. 2014. Long-term copper partitioning of metal-spiked sediments used in outdoor mesocosms. Environmental Science and Pollution Research 21:7130-7139.
1177.2 Estuarine Health Assessment - Experiment - Published 29 Sep 2014
125 samples of benthic eukaryotic communities from SE Queensland. Samples were collected from the Noosa, Maroochydore, Pine, Logan and Currumbin estuaries in February 2010. Five sites per estuary, wit... moreh 5 samples per site.
18S rRNA using the following primers:
All18SF-TGGTGCATGGCCGTTCTTAGT and All18SR-CATCTAAGGGCATCACAGACC
1177.2 Estuarine Health Assessment - Experiment - Published 22 Jan 2014
Roche 454 FLX Titanium produced 18S rDNA amplicons derived from an experiment which examined the vertical migration of benthic biota through triclosan contaminated surficial sediments.
The primers u... moresed were 18S SSU_F04 (5’-GCTTGTCTCAAAGATTAAGCC-3’) and 18S SSU_R22 (5’-GCCTGCTGCCTTCCTTGGA-3’)
The study contained four treatments The treatments were: 1) Field Control with no sediment added; 2) Triclosan Control with reference sediment added from Long Island Sound (LIS); 3) Low Triclosan (30 mg triclosan/kg dry LIS sediment); and 4) High Triclosan (300 mg triclosan/kg dry LIS sediment ).
1177.2 Estuarine Health Assessment - Experiment - Published 11 Dec 2012