Showing results for: [ next generation sequencing ]
We describe two versions of COBRA-seq: First, Genome-Wide COBRA (GW-COBRA) that uses PCR to exponentially amplify the sequencing library fragments after linker ligation. Second, Linear Amplification C... moreOBRA (LA-COBRA) that relies on T7 RNA polymerase-mediated transcription yielding many RNA transcripts of the library fragments generated in a linear manner which are subsequently converted to cDNA. The COBRA-seq library protocol is largely adapted to provide robust coverage of the human genome, however, all of the steps are applicable to both model and non-model organisms.
SIEF: Nutritional Genomics - COBRA-seq: Sensitive and Quantitative Methylome Profiling - Published 29 Sep 2015
Raw sequencing reads and final assembled contigs associated with the 454 pyrosequencing of the stressor associated transcriptome of the gill and digestive gland of the Sydney Rock Oyster (Saccostrea g... morelomerata). Original .fasta and .qual files are included, as well as the final assembly as described in the paper (Hook et al., Marine Genomics, to be submitted).less
1177.2 Estuarine Health Assessment - Experiment - Published 15 Aug 2014
Total RNA extracted from liver from 12 individuals and pooled in equal quantities. Individual RNA was from BAR 10-3 timecourse post feeding experiment. Liver RNA extracted from different times after f... moreeeding.
Pooled animals: 2 animals (T0-J and K) - not fed; 3 animals; 1hr (T60-I,J,K) and 2hrs (T120-I,J,K) after feeding (6 total); 1 animal - 4hrs (T4-I), 8hrs (T8-I), 12hrs (T12-I) and 24hrs (T24-I) after feeding
Generated thousands of unique barramundi sequences for gene discovery in fastq format. Used for de novo transcriptome assembly for barramundi liver.
Lab Notebook: LN2010/1717 p85less
CLSD Feed Tech Novel Feeds - Transcriptomics - Published 16 Aug 2013