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Outdoor freshwater mesocosms spiked with varying concentrations of copper were sampled in order to profile microbial communities. Samples collected include water, sediment and cellulose baits. DNA was... more extracted from all sample types. In addition to this, RNA was extracted from sediment samples and reverse transcribed to get cDNA. The v4 region of the 16S ribosomal RNA gene was amplified using Earth Microbiome primers 515F and 806R.
The 16S rRNA gene amplicons were sequenced on the Illumina MiSeq platform with 250 base pair-end reads. Forward and reverse read alignments, quality filtering, chimera checking and OTU clustering were performed using USEARCH v9.
OTU sequences were submitted to GenBank, however, 130 were found to be too novel for submission and thus are made available here.less
Legacy data - Microbial community survey - Published 10 Oct 2017
Draft genome assemblies of 380 Bradyrhizobium isolates collected from soil samples at the base of Acacia acuminata trees in Southwest Australia
Biodiversity Eco Know & Serv - Awards & - Survey of rhizobia in soils of South-West Australia - Published 03 Mar 2017
Sphingobium yanoikuyae strain_B2_R1_001.fastq.gz
Sphingobium yanoikuyae strain_B2_R2_001.fastq.gz
*Velvet assembly as scaffolds or contigs*
*Annotation files - based on scaffolds*
Annotation Sphingobium yanoikuyae strain B2.faa
Annotation Sphingobium yanoikuyae strain B2.gff
*Putative cytochrome sequences*
*Putative plasmid sequence*
Sphingobium yanoikuyae strain B2 putative plasmid.zip - file contains the fasta, gene calls and BLAST matches for this contigless
Industrial Biotechnology - Stratetic Pro - Bacterial genomics - Published 19 Jul 2016
This software accompanies the publication of the paper "Spotsizer: High through-put quantitative analysis of microbial growth" by Leanne Bischof et al. It contains the software version that is referre... mored to in that publication.less
Legacy data - Spotsizer development - Published 10 May 2016
The Wildlife Parasite and Pathology Collection is a biodiversity resource backing up nearly 40 years of research into the biology and taxonomy of Australian native and introduced parasites and the bio... morelogy and pathology of their hosts.
The Collection currently holds 9,228 irreplaceable specimens of 1074 parasite species, covering 94 families in 13 orders, from 7,358 individual postmortems on 579 host species. The collection documents 24,110 individual host-parasite associations, representing 3,307 unique associations between a given parasite species and a given host species.
The Collection focuses on the parasites of the terrestrial vertebrates of Australia and Papua New Guinea and rodents of South-East Asia. There are also specimens from other parts of the world.less
Australian National Wildlife Collection - Biological Specimen Collections - Published 24 Jan 2013