Raw reads and final assembled contigs for Melita project

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About this Collection

Raw reads and final assembled contigs for Melita project

Raw sequencing reads and final assembled contigs associated with the 454 sequencing of the toxicant responsive transcriptome of the amphipod Melita plumulosa. Original .fasta and .qual files are included, as well as the contigs associated with the final assembly as desribed in the paper (Hook et al., to be submitted, BMC Genomics)

Environmental Sciences not elsewhere classified

CSIRO Enquiries
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transcriptome amphipods sequencing reads contigs


Galaxy workflows, experimental design, etc

The reads are the output of 454 pyrosequencing runs performed at the Ramaciotti Centre, UNSW. The contigs were assembled by intitally filtereing the reads for quality using the CSIRO Galaxy instance then by assembling the reads using the MIRA algorithm and the CSIRO Galaxy instance.

Sharon E. Hook and Stuart L Simpson conceived of and designed the study; Sharon E Hook, Stuart L Simpson , and Marc Wilkins (UNSW) obtained funding; David A Spadaro conducted culturing, toxicological exposures and animal collection; Sharon E Hook conducted molecular biological laboratory work; Sharon E Hook, Natalie Twine (UNSW), Philippe Moncuquet and Marc Wiilkins analyzed the data; Sharon E Hook, Stuart L Simpson, Natalie Twine, Philippe Moncuquet and Marc Wilkins interpreted the data; all authors contributed to writing the manuscript. All authors read and approved the final manuscript.

Creative Commons Attribution 3.0 Unported Licence

CSIRO (Australia), University of New South Wales (Australia)

Hook, Sharon; Twine, Natalie; Simpson, Stuart; Spadaro, David; Moncuquet, Philippe; Wilkins, Marc (2013): Raw reads and final assembled contigs for Melita project. v2. CSIRO. Data Collection.

All Rights Reserved (including copyright) CSIRO Australia, University of New South Wales 2013.

The metadata and files (if any) are available to the public.

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About this Project

1177.2 Estuarine Health Assessment

Metabarcoding of Copper Contaminated Sediments

Sharon Hook


Manipulative experiment using whole benthic communities obtained from an estuary in Rhode Island, USA.


Roche 454 FLX Titanium

Sharon Hook

Natalie Twine

Stuart Simpson

David Spadaro

Philippe Moncuquet

Marc Wilkins

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