Transcriptome assembly of Chinese tallow (Triadica sebifera)

CLSD EF-RP3 CDF25.1 CESRE TSOP 2013/2014

PROJECT TITLE Understanding tallow tissue development: defining regulatory gene networks for non-seed lipid accumulating tissues. DESCRIPTION Oil in the form of triacylglycerol (TAG) is the major storage compound for fatty acids (FAs) and is the most energy-dense storage form of reduced carbon in many species (Durrett et al., 2008). Unlike most pl... moreants that accumulate oil in seed tissues, some species accumulate large amounts of TAG in other tissues, like the mesocarp of oil palm, avocado and olive. However, it is important to develop crops that will have minimal impact on world food supply and agricultural land, to be considered viable as alternative fuel. In this project, we study the TAG accumulation pathways of Chinese tallow tree (CT), Triadica sebifera, a valuable oil-producing tree that can grow in a variety of conditions without competing for food production (Jeffrey and Padley, 1991). The non-seed tallow layer of CT is known to accumulate more lipid than the kernel tissue and is rich in palmitic acid (16:0) and oleic acid (18:1), whereas the kernel is rich in linoleic and gamma-linolenic acid (Picou and Boldor, 2012). We deposit the transcriptome data here which is the first resource of any kind for this valuable crop accumulating high amounts of oil in both seed and non-seed tissues. less

Surinder Singh

Annotation and Taxnomic distribution of Chinese tallow (Triadica sebifera) transcriptome

Since Ricinus communis was the most closely related species, we used its annotated transcriptome as a reference to identify Triadica sebifera homologs. A database was generated with the R. communis transcript sequences downloaded from PlantGDB (http://www.plantgdb.org/RcGDB/) and blasted against the T. sebifera transcriptome. The T. sebifera seque... morences mapped to R. communis genes are described less

Analysis

Uday Divi