Bacterial Communities of S Alligator River Floodplain (Kakadu)

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About this Collection

Bacterial Communities of S Alligator River Floodplain (Kakadu)

Bacterial survey of the S Alligator River Floodplain (Kakadu). The study was designed to examine how history to saltwater exposure alters microbial assemblages. The research was part of the NERP Northern Australian Hub. Data includes 90 samples taken from three different areas of energy (low, medium and high) and three areas of the floodplain (l... more

Environmental Monitoring

29 Aug 2012

30 Aug 2012

CSIRO Enquiries
1300 363 400

bateria floodplain saltwater intrusion 16S rRNA metabarcoding Kakadu

Oligonucleotide barcoded primers were used to PCR amplify a ~600 base pair (bp) product spanning the V4 and V6 hyper variable region of the 16S rRNA gene 563F, 5’-ccatctcatccctgcgtgtctccgactcag-AYTGGGYDTAAAGNG-3’ (Claesson, et al., 2010) and 1046R, 5’-cctatcccctgtgtgccttggcagtctcag-CGACAGCCATGCANCACCT- 3’ (Sogin, et al., 2006). Data were analysed using the Mothur v.1.30.1. Sequences were aligned to the SILVA database (Pruesse et al. 2007) and those that did not align were removed. Alignments were trimmed so that all sequences covered the entire alignment length. Sequences were clustered at a similarity cut-off of 97%. Representatives of resultant operational taxonomic units (OTUs) were identified using the SILVA taxonomy tool. The final dataset comprised 939,511 sequences.

This research was funded under the NERP Northern Australia Hub, Project 5.4. The research was a collaboration between the Federal Government, CSIRO, AIMS and Charles Darwin University.

CSIRO Data Licence

Australian Institute of Marines Sciences (Australia), Charles Darwin University (Australia), CSIRO (Australia), Department of the Enviroment (Australia)

CSIRO; Streten, Claire; Gibb, Karen (2015): Bacterial Communities of S Alligator River Floodplain (Kakadu). v1. CSIRO. Data Collection.

All Rights (including copyright) Australian Institute of Marines Sciences, Charles Darwin University, CSIRO Australia, Department of the Enviroment 2015.

The metadata and files (if any) are available to the public.

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Location Details

12°22′10″ S

12°46′10″ S

132°33′30″ E

132°21′30″ E


About this Project

1177.2 Estuarine Health Assessment

Metabarcoding of Copper Contaminated Sediments

Anthony Chariton


Manipulative experiment using whole benthic communities obtained from an estuary in Rhode Island, USA.


Roche 454 FLX Titanium


Claire Streten

Karen Gibb

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