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Raw reads and final assembled contigs for pennate diatom project

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About this Collection

Raw reads and final assembled contigs for pennate diatom project


Raw sequencing reads and final assembled contigs associated with the 454 sequencing of the toxicant responsive transcriptome of the diatom Ceratoneis closterium. This species often is also often referred to by its previous names, Nitzschia closterium and Cylindrotheca closterium. It is species CS-5 in the CSIRO Collection of Living Microalgae, Mari... more


Environmental Science and Management not elsewhere classified


https://doi.org/10.4225/08/525BCD6E6F145


CSIRO Enquiries
CSIROEnquiries@csiro.au
1300 363 400

Phytoplankton ecotoxicology photosynthesis Next generation sequencing gene expression stress response


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Link to Galaxay pipelines


The reads are the output of 454 pyrosequencing runs performed at the Ramaciotti Centre, UNSW. The contigs were assembled by intitally filtereing the reads for quality using the CSIRO Galaxy instance then by assembling the reads using the MIRA algorithm and the CSIRO Galaxy instance.


Sharon E. Hook and Merrin S Adams conceived of and designed the study; Sharon E Hook, Merrin S Adams, and Marc Wilkins (UNSW) obtained funding; Francesca Gissi and Hannah L Osborn conducted culturing, toxicological exposures and algal collection;Hannah L Osborn conducted molecular biological laboratory work; Sharon E Hook, Natalie Twine (UNSW), Philippe Moncuquet and Marc Wiilkins analyzed the data; Sharon E Hook, Merrin S Adams, Hannah L Osborn, Natalie Twine, Philippe Moncuquet and Marc Wilkins interpreted the data; all authors contributed to writing the manuscript. All authors read and approved the final manuscript.


Creative Commons Attribution 3.0 Unported Licence


CSIRO (Australia)


Hook, Sharon; Osborn, Hannah; Gissi, Francesca; Moncuquet, Philippe; Twine, Natalie; Wilkins, Marc; Adams, Merrin (2013): Raw reads and final assembled contigs for pennate diatom project. v1. CSIRO. Data Collection. https://doi.org/10.4225/08/525BCD6E6F145


All Rights (including copyright) CSIRO Australia 2013.


The metadata and files (if any) are available to the public.

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About this Project

1177.2 Estuarine Health Assessment


Metabarcoding of Copper Contaminated Sediments


Sharon Hook


Experiment


Manipulative experiment using whole benthic communities obtained from an estuary in Rhode Island, USA.


Experiment


Roche 454 FLX Titanium


Sharon Hook


Hannah Osborn


Francesca Gissi


Philippe Moncuquet


Natalie Twine


Marc Wilkins


Merrin Adams


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