Raw reads and final assembled contigs for pennate diatom project

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About this Collection

Raw reads and final assembled contigs for pennate diatom project

Raw sequencing reads and final assembled contigs associated with the 454 sequencing of the toxicant responsive transcriptome of the diatom Ceratoneis closterium. This species often is also often referred to by its previous names, Nitzschia closterium and Cylindrotheca closterium. It is species CS-5 in the CSIRO Collection of Living Microalgae, Mari... more

Environmental Science and Management not elsewhere classified

CSIRO Enquiries
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Phytoplankton ecotoxicology photosynthesis Next generation sequencing gene expression stress response


Link to Galaxay pipelines

The reads are the output of 454 pyrosequencing runs performed at the Ramaciotti Centre, UNSW. The contigs were assembled by intitally filtereing the reads for quality using the CSIRO Galaxy instance then by assembling the reads using the MIRA algorithm and the CSIRO Galaxy instance.

Sharon E. Hook and Merrin S Adams conceived of and designed the study; Sharon E Hook, Merrin S Adams, and Marc Wilkins (UNSW) obtained funding; Francesca Gissi and Hannah L Osborn conducted culturing, toxicological exposures and algal collection;Hannah L Osborn conducted molecular biological laboratory work; Sharon E Hook, Natalie Twine (UNSW), Philippe Moncuquet and Marc Wiilkins analyzed the data; Sharon E Hook, Merrin S Adams, Hannah L Osborn, Natalie Twine, Philippe Moncuquet and Marc Wilkins interpreted the data; all authors contributed to writing the manuscript. All authors read and approved the final manuscript.

Creative Commons Attribution 3.0 Unported Licence

CSIRO (Australia)

Hook, Sharon; Osborn, Hannah; Gissi, Francesca; Moncuquet, Philippe; Twine, Natalie; Wilkins, Marc; Adams, Merrin (2013): Raw reads and final assembled contigs for pennate diatom project. v1. CSIRO. Data Collection.

All Rights (including copyright) CSIRO Australia 2013.

The metadata and files (if any) are available to the public.

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About this Project

1177.2 Estuarine Health Assessment

Metabarcoding of Copper Contaminated Sediments

Sharon Hook


Manipulative experiment using whole benthic communities obtained from an estuary in Rhode Island, USA.


Roche 454 FLX Titanium

Sharon Hook

Hannah Osborn

Francesca Gissi

Philippe Moncuquet

Natalie Twine

Marc Wilkins

Merrin Adams

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