Processing...

Barramundi Liver Raw Sequence Reads

Tools Click here to view this collection in the new DAP user interface

show summary fields  |   show all    
Tools



About this Collection

Barramundi Liver Raw Sequence Reads


Total RNA extracted from liver from 12 individuals and pooled in equal quantities. Individual RNA was from BAR 10-3 timecourse post feeding experiment. Liver RNA extracted from different times after feeding. Pooled animals: 2 animals (T0-J and K) - not fed; 3 animals; 1hr (T60-I,J,K) and 2hrs (T120-I,J,K) after feeding (6 total); 1 animal - 4hrs (T... more


Animal Cell and Molecular Biology Gene Expression (incl. Microarray and other genome-wide approaches)


https://doi.org/10.25919/5c36d3ca61dad


07 Aug 2013


07 Aug 2013


CSIRO Enquiries
CSIROEnquiries@csiro.au
1300 363 400

next generation sequencing Roche 454 GLX Titanium transcriptome


Sample Name: 2011_04_25_NickWade_Fish_Liver Application: Transcirptome Sequencing Sample Buffer: water 260/280 ratio: 2.0 Conc (ng/ul): 500 Volume (ul): 480 Total Amount: 240ug Sample Type: Total RNA Library Type: Shotgun library Pooling: equal amounts (20ug) from 12 individuals Machine: GS-FLX Titanium Run type: Normal Run Scale: 1/4 region Barcode sorting: no Read count: 109,883 Total bases: 38,088,470 Av Read length: 300bp File Format: FASTQ (.fa) contains nucleotide sequence and corresponding quality scores


All experimental animal samples, RNA extractions and quality assessments, sample preparation and data generation was performed by Nick Wade.


Creative Commons Attribution 3.0 Unported Licence


CSIRO (Australia)


Wade, Nick; Glencross, Brett (2013): Barramundi Liver Raw Sequence Reads. v1. CSIRO. Data Collection. 102.100.100/13190 https://doi.org/10.25919/5c36d3ca61dad


All Rights (including copyright) CSIRO Australia 2013.


Although the associated metadata is public, the files (if any) have not been approved for general release. Please phone or email the contact person for this collection to discuss access to the files.

show all

About this Project

CLSD Feed Tech Novel Feeds


Expansion of genetic resources in the Giant Tiger Shrimp, Penaeus monodon


Nick Wade


Transcriptomics


Shotgun sequencing of RNA to identify novel sequence information. Likely pooled sample from a number of different developmental stages or time points.


Experiment


any next generation machine such as Illumina, 454, Ion Torrent etc


Nick Wade


Brett Glencross