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Barley Genotype: presence of VERNALIZATION1 and VERNALIZATION2 genes in varieties and landraces of Barley.

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About this Collection

Barley Genotype: presence of VERNALIZATION1 and VERNALIZATION2 genes in varieties and landraces of Barley.


Identification of presence of the VERNALIZATION1 (VRN1) and VERNALIZATION2 (VRN2) novel alleles in varieties and landraces of Barley seed from the Australian Winter Cereal Collection (AWCC). The data was collected 2005-06. See Hemming MN, Fieg S, Peacock WJ, Dennis ES and Trevaskis B. 2009. Regions associated with repression of the barley (Hordeum ... more


Crop and Pasture Improvement (Selection and Breeding)


https://doi.org/10.4225/08/506E68D6BAF02


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CSIROEnquiries@csiro.au
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Barley Cereals flowering vernalization VRN1 VRN2


:

Hemming MN, Fieg S, Peacock WJ, Dennis ES and Trevaskis B. 2009. Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Molecular Genetics and Genomics. 282: 107-117


Please see the included DNA extraction protocol. See Hemming MN, Fieg S, Peacock WJ, Dennis ES and Trevaskis B. 2009. Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Molecular Genetics and Genomics. 282: 107-117. for other method details and key to results. Please note that the data is preliminary, as some seeds are not homogenous. “Landraces” were frequently found to have mixed genotypes for VRN1 . The same is true for some modern varieties. Additionally, technical and operator error will reduce the accuracy of our genotyping data. Finally, other genes (eg. VRN3 ), which have not been examined, might also influence flowering behaviour. Despite these limitations, the genotype data does allow selection for particular flowering behaviour. For example, a large number of potential winter wheats can now be selected from amongst the AWCC collection. Similarly, large numbers of facultative barleys (early flowering in long days) could be identified using these genotype data. We would recommend subsequent flowering time tests or re-genotyping (multiple seeds per line, or single seed descent) to confirm our initial genotype scores.


Creative Commons Attribution ShareAlike 3.0 Unported Licence


CSIRO (Australia), Grains Research and Development Corporation (Australia)


Trevaskis, Ben; Fieg, Sarah (2012): Barley Genotype: presence of VERNALIZATION1 and VERNALIZATION2 genes in varieties and landraces of Barley.. v1. CSIRO. Data Collection. https://doi.org/10.4225/08/506E68D6BAF02


All rights (including copyright) CSIRO Australia, Grains Research and Development Corporation 2012.


The metadata and files (if any) are available to the public.

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About this Project

CLSD, GRDC, CSP00131, Finding the balanc


R-00165-15-011


Ben Trevaskis


Genome screening in seeds


DNA extraction and screening for specific alleles


Measurement


Ben Trevaskis


Sarah Fieg


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