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Genetic mapping and transcriptomic characterization of a new fuzzless-tufted cottonseed mutant
fuzzless-tufted upland cotton mutant (Gossypium hirsutum) and control at 4 time points (0DPA, 2DPA, 4DPA, 6DPA)
Crop and Pasture Biochemistry and Physiology
Crop and Pasture Improvement (Selection and Breeding)
Cotton Fuzzless Mutant Transcriptomic
CSIRO Data Licence
Moncuquet, Philippe (2020): Genetic mapping and transcriptomic characterization of a new fuzzless-tufted cottonseed mutant. v1. CSIRO. Data Collection.
All Rights (including copyright) CSIRO 2020.
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CBA04: DR - Marker Technology
Fibreless mutants are unique and valuable resources for understanding the genetic and molecular mechanisms controlling initiation and development of cotton fibres that are extremely elongated single epidermal cells arising from the seed coat of cottonseeds. In this study, we investigated the genetics of a new fuzzless-tufted upland cotton mutant (G... moreossypium hirsutum) and found that the mutant phenotype is caused by a single mutation of incomplete dominance, designed N5. The mutation was mapped to an approximately 250-kb interval containing 33 annotated genes using a combination of bulked segregant sequencing, SNP chip genotyping, and fine mapping. Comparative transcriptomic analysis using 0 to 6 days-post-anthesis (dpa) ovules from near-isogenic lines (NILs) segregating for the phenotypes of fuzzlss-tufted and normal fuzzy cottonseeds uncovered candidate genes responsible for the mutant phenotype. It also revealed that the flanking region of the N5 locus is enriched for genes differentially expressed between the mutant and its NILs with normal fuzzy cottonseeds. Several of those differentially expressed genes (DEGs) are members of the gene families, such as calcium dependent protein kinase and expansin, with demonstrated roles in cell initiation and elongation. The transcriptome landscape of the mutant is significantly reprogrammed in the 6 dpa ovules and, to a less extent, in the 0 dpa ovules, but not in the 2 and 4 dpa ovules. The changes were mainly observed in genes associated with cell wall modifications and transmembrane transportation (in 0 and 6 dpa ovules) as well as in transcription factors (in 6 dpa ovules). The transcriptome changes are in agree with the fibre phenotype of the mutant. These results imply a similar molecular basis for initiation of lint and fuzz fibres. less