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Proteomics Data: Hordein accumulation in developing barley grains

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About this Collection

Proteomics Data: Hordein accumulation in developing barley grains


Proteomics data collected as part of the manuscript by Tanner et al (2019) Frontiers in Plant Science Abstract: The temporal pattern of accumulation of hordein storage proteins in developing barley grains was studied by enzyme-linked immunosorbent assay (ELISA), western blot and liquid chromatography tandem mass spectrometry (LC-MS/MS). Hordein acc... more


Analytical Spectrometry Crop and Pasture Biochemistry and Physiology Proteins and Peptides Proteomics and Intermolecular Interactions (excl. Medical Proteomics)


https://doi.org/10.25919/5ca3ce21e83f8


Michelle Colgrave
Michelle.Colgrave@csiro.au

gluten hordein expression proteomics barley grain multiple reaction monitoring mass spectrometry (MRM-MS)


Proteins extracted from developing barley grain, digested with either trypsin or chymotrypsin. Peptides were analysed by LC-MS on a 6500QTRAP LC-MS system. Raw data deposited in .wiff/.wiff.scan format.


Gregory J. Tanner produced the grain samples. Malcolm J. Blundell grew and milled grain. Michelle L. Colgrave performed sample preparation and data acquisition.


Creative Commons Attribution 4.0 International Licence


CSIRO (Australia), The University of Melbourne (Australia), La Trobe University (Australia)


Colgrave, Michelle; Tanner, Gregory; Blundell, Malcolm; Howitt, Crispin; Bacic, Antony (2019): Proteomics Data: Hordein accumulation in developing barley grains. v2. CSIRO. Data Collection. https://doi.org/10.25919/5ca3ce21e83f8


All Rights (including copyright) CSIRO 2019.


The metadata and files (if any) are available to the public.

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About this Project

Livestock - Testing & Services


Mass spectrometry data collected for gluten project (Greg Tanner)


Drewe Ferguson





Michelle Colgrave


Gregory Tanner


Malcolm Blundell


Crispin Howitt


Antony Bacic


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