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National Livestock Methane Program Project B.CCH.6610 / 01200.038
Data was generated for NLMP project entitled "Culture independent metagenomic approaches for understanding the functional metabolic potential of methanogen communities in ruminant livestock"
Animal Production not elsewhere classified
Veterinary Microbiology (excl. Virology)
Datasets encompass multiple 'omics approaches to characterise methanogen communities in ruminant livestock; including 16S rRNA amplicon sequencing; in vitro transcriptomics (Methanobrevibacter ruminantium M1); targeted metagenomics (to enrich for Methanobrevibacter and Methanosphaera genomes); and metatranscriptomics
This project was supported by funding from Meat & Livestock Australia; and the Australian Government Department of Agriculture as part of the Carbon Farming Futures Filling the Research Gap Program
CSIRO Data Licence
Australian Government Department of Agriculture (Australia), CSIRO (Australia), James Cook University (Australia), Meat And Livestock Australia (Australia), University of Western Australia (Australia)
CSIRO (2015): National Livestock Methane Program Project B.CCH.6610 / 01200.038. v1. CSIRO. Data Collection. 102.100.100/22181
All Rights (including copyright) CSIRO Australia 2015.
Although the associated metadata is public, the files (if any) have not been approved for general release. Please phone or email the contact person for this collection to discuss access to the files.
Metagenomic approaches for understanding
Many surveys continue to be undertaken with the aim of cataloguing the key microbes responsible for methane production in livestock (“who’s there?”), but these studies have been unable to provide functional information to describe their behaviour in the rumen (“what are they doing?”). There is a paucity of knowledge about how the genetic potential... more of methanogens is expressed to support their growth and (or) methane producing activity. By recovering, examining and comparing the genomes and gene expression profiles of rumen methanogens from Australian production systems, we have been able to reveal mechanisms that are critical for their persistence in vivo. This information has filled a significant research gap and can now be translated into a precise focus (rather than an empirical “shotgun” approach) for development of targeted approaches for inhibition of methanogenesis in ruminants. less
Rumen Methanobacteriales metagenomes
Bovine rumen samples were enriched in the lab to increase the representation of DNA from members of the order Methanobacteriales
Illumina HiSeq 2000
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