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National Livestock Methane Program Project B.CCH.6610 / 01200.038

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About this Collection

National Livestock Methane Program Project B.CCH.6610 / 01200.038


Data was generated for NLMP project entitled "Culture independent metagenomic approaches for understanding the functional metabolic potential of methanogen communities in ruminant livestock"


Animal Production not elsewhere classified Veterinary Microbiology (excl. Virology)


https://doi.org/10.25919/5c36d82fac61c


2012


2015


CSIRO Enquiries
CSIROEnquiries@csiro.au
1300 363 400

rumen methane microbiology transcriptome metatranscriptome metagenome Methanobrevibacter Methanosphaera


Fact_sheet.pdf.pdf


Datasets encompass multiple 'omics approaches to characterise methanogen communities in ruminant livestock; including 16S rRNA amplicon sequencing; in vitro transcriptomics (Methanobrevibacter ruminantium M1); targeted metagenomics (to enrich for Methanobrevibacter and Methanosphaera genomes); and metatranscriptomics


This project was supported by funding from Meat & Livestock Australia; and the Australian Government Department of Agriculture as part of the Carbon Farming Futures Filling the Research Gap Program


CSIRO Data Licence


Australian Government Department of Agriculture (Australia), CSIRO (Australia), James Cook University (Australia), Meat And Livestock Australia (Australia), University of Western Australia (Australia)


CSIRO (2015): National Livestock Methane Program Project B.CCH.6610 / 01200.038. v1. CSIRO. Data Collection. 102.100.100/22181 https://doi.org/10.25919/5c36d82fac61c


All Rights (including copyright) CSIRO Australia 2015.


Although the associated metadata is public, the files (if any) have not been approved for general release. Please phone or email the contact person for this collection to discuss access to the files.

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About this Project

Metagenomic approaches for understanding


Many surveys continue to be undertaken with the aim of cataloguing the key microbes responsible for methane production in livestock (“who’s there?”), but these studies have been unable to provide functional information to describe their behaviour in the rumen (“what are they doing?”). There is a paucity of knowledge about how the genetic potential... more


Carly Rosewarne


Rumen Methanobacteriales metagenomes


Bovine rumen samples were enriched in the lab to increase the representation of DNA from members of the order Methanobacteriales


Analysis


Illumina HiSeq 2000


CSIRO


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